FCM has until recently been predominantly used to phenotype leukemias and lymphomas and to aid in prognosis of nonhematologic tumors. Nonhematologic tumors In nonhematologic tumors, predominately aneuploid neoplasms (especially if the S-phase value is increased) generally are more aggressive and have shorter survival time than tumors that are predominantly diploid and have normal S-phase values. […]
Considerably simplified, flow cytometry (FCM) counts and analyzes certain aspects of cells using instrumentation similar in principal to many current hematology cell-counting machines. If the cells to be analyzed come from solid tissue, the cells or cell nuclei must first be extracted from the tissue and suspended in fluid. Next, the cell nuclei are stained […]
WBC identification is usually done by Wright-stained peripheral blood smear examination. However, this approach creates problems due to the statistically small number of cells counted (usually 100), nonuniform cell distribution on the smear, and the need for subjective interpretation that can produce differences in cell counts in the same smear by the same technologist or […]
